Extraction and purification of magnetic nanoparticles from strain of Leptospirillum ferriphilum

来源期刊：中国有色金属学报(英文版)2006年第6期

论文作者：高健 谢建平 丁建南 康健 程海娜 邱冠周

文章页码：1417 - 1420

Key words：Leptospirillum ferriphilum; strain YSK; SDS; sucrose density gradient; magnetosomes; magnetotaxis

Abstract: The magnetic nanoparticles were extracted from Leptospirillum ferriphilum, strain YSK, isolated from acid mine drainages by treatment with sodium dodecyl sulfate(SDS) and centrifugation through a sucrose density gradient. Transmission electron microscopy(TEM) indicates that the nanoparticles are approximately spherical with a mean diameter of 44 nm, and magnetite crystals in this size range are single magnetic domains. Energy-dispersive X-ray analysis shows that the nanoparticles primarily contain two kinds of elements, iron and oxygen. Thus it can be concluded that the magnetic particles are magnetosomes. Generally, it is thought that cellular magnetotaxis is a direct consequence of the cell possessing magnetosomes. The discovery of magnetosomes in strain YSK can provide the theoretical basis for screening efficient bioleaching bacteria which are specific to different magnetic minerals under an outer magnetic field.

基金信息：the Aviation Science Foundation of China
the National Natural Science Foundation of China