Effect of Rare Earth Ions on Kinetic Properties of Escherichia coli Leucyl-tRNA Synthetase
来源期刊：JOURNAL OF RARE EARTHS2000年第3期
论文作者：TU Hua-min WANG En-duo LI Yong YANG Yan-sheng
Key words：rare earths; leucyl-tRNA synthetase; aminoacylation;
Abstract: Escherichia coli leucyl-tRNA synthetase (LeuRS) is one of aminoacyl-tRNA synthetases (aaRSs) and belongs to class 1 aaRSs. The apparent steady-state kinetics of the aminoacylation reaction catalyzed by LeuRS in the presence of some RE3+ were studied. The results show that Mg2+ can be substituted by RE3+ for the aminoacylation reaction. The apparent Km values for ATP and leucine are markedly different between native and Mg2+-free tRNA1Leu. At high concentration of ATP there is inhibitory effect on Mg2+-free tRNA but not on native tRNA, which indicates that metal ions are a substrate of the aminoacylation reaction.
TU Hua-min1，WANG En-duo2，LI Yong2，YANG Yan-sheng3
(1.Department of Chemistry, Hebei Normal University, Shijiazhuang 050091, China;
2.State-Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai 200031, China;
3.Department of Chemistry, Zhongshan University, Guangzhou 510275, China)
Abstract:Escherichia coli leucyl-tRNA synthetase (LeuRS) is one of aminoacyl-tRNA synthetases (aaRSs) and belongs to class 1 aaRSs. The apparent steady-state kinetics of the aminoacylation reaction catalyzed by LeuRS in the presence of some RE3+ were studied. The results show that Mg2+ can be substituted by RE3+ for the aminoacylation reaction. The apparent Km values for ATP and leucine are markedly different between native and Mg2+-free tRNA1Leu. At high concentration of ATP there is inhibitory effect on Mg2+-free tRNA but not on native tRNA, which indicates that metal ions are a substrate of the aminoacylation reaction.
Key words:rare earths; leucyl-tRNA synthetase; aminoacylation;
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