Immobilization of RGD Peptide onto the Surface of Apatite-Wollastonite Ceramic for Enhanced Osteoblast Adhesion and Bone Regeneration
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2014年第3期
论文作者：张翔 GU Jianwen ZHANG Yue TAN Yanfei ZHOU Jiabei 周大利
文章页码：626 - 634
摘 要：The arginine-glycine-aspartic（RGD） acid peptide was grafted to the surface of apatitewollastonite（AW） ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalently immobilized onto the surface of AW ceramic via the synthetic cross linker AAPTS-E and 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide（EDC）. The modified surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy（ATR-FTIR） and X-ray photoelectron spectroscopy（XPS）. The chemical analysis indicated that RGD peptide had been immobilized onto the AW surface successfully. The growth of osteoblast-like cells（MG63） showed that modifying the AW surface with RGD peptide enhanced the cell adhesion and proliferation. And the histological evaluation of RGD-AW showed that the bone regeneration and remodeling process were signifi cantly enhanced compared to the original AW ceramics after 2, 4 and 8 weeks implantation in rabbit’s femoral condyles.
张翔1，GU Jianwen1,2，ZHANG Yue3，TAN Yanfei1，ZHOU Jiabei1，周大利1
1. College of Materials Science and Engineering, Sichuan University2. The Military General Hospital of Chengdu PLA3. The First Affiliated Hospital of Gannan Medical University
摘 要：The arginine-glycine-aspartic（RGD） acid peptide was grafted to the surface of apatitewollastonite（AW） ceramic in an effort to improve its cell adhesion, proliferation and osteoinduction. RGD peptide was covalently immobilized onto the surface of AW ceramic via the synthetic cross linker AAPTS-E and 1-ethyl-3-（3-dimethylaminopropyl）carbodiimide（EDC）. The modified surfaces were characterized by attenuated total reflectance Fourier transform infrared spectroscopy（ATR-FTIR） and X-ray photoelectron spectroscopy（XPS）. The chemical analysis indicated that RGD peptide had been immobilized onto the AW surface successfully. The growth of osteoblast-like cells（MG63） showed that modifying the AW surface with RGD peptide enhanced the cell adhesion and proliferation. And the histological evaluation of RGD-AW showed that the bone regeneration and remodeling process were signifi cantly enhanced compared to the original AW ceramics after 2, 4 and 8 weeks implantation in rabbit’s femoral condyles.
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