Characteristics of magnetic Fe₃O₄ nanoparticles encapsulated with human serum albumin

来源期刊：中南大学学报(英文版)2006年第1期

论文作者：何捍卫 刘红江 周科朝 王维 容鹏飞

文章页码：6 - 11

Key words：Fe₃O₄ nanoparticle; human serum albumin; chemical precipitation method; magnetic resonance imaging contrast agent

Abstract: Magnetic nanoparticles (Fe₃O₄) were prepared by chemical precipitation method using Fe²⁺and Fe³⁺ salts with sodium hydroxide in the nitrogen atmosphere. Fe₃O₄ nanoparticles were coated with human serum albumin(HSA) for magnetic resonance imaging as contrast agent. Characteristics of magnetic particles coated or uncoated were carried out using scanning electron microscopy and X-ray diffraction. Zeta potentials, package effects and distributions of colloid particles were measured to confirm the attachment of HSA on magnetic particles. Effects of Fe₃O₄ nanoparticles coated with HSA on magnetic resonance imaging were investigated with rats. The experimental results show that the adsorption of HSA on magnetic particles is very favorable to dispersing of magnetic Fe₃O₄ particles, while the sizes of Fe₃O₄ particles coated are related to the molar ratio of Fe₃O₄ to HSA. The diameters of the majority of particles coated are less than 100 nm. Fe₃O₄ nanoparticle coated with HSA has a good biocompatibility and low toxicity. This new contrast agent has some effects on the nuclear magnetic resonance imaging of liver and the lowest dosage is 20μmol/kg for the demands of diagnosis.