Amperometric glucose biosensor based on gold nanorods and chitosan comodified Au electrode
来源期刊：Rare Metals2010年第3期
论文作者：LI Yingyinga,b,WANG Xiaoxiaa,DONG Xianga,HE Zhipenga,and ZHANG Haiqiana,c a College of Material Science and Technology,Nanjing University of Aeronautics and Astronautics,Nanjing ,China b Kunming Fire Service Training School,Kunming ,China c Jiangsu Laboratory for Biomaterials and Devices,Southeast University,Nanjing ,China
文章页码：238 - 242
摘 要：Au nanorods were prepared by a seeding growth approach and used in fabricating the nanorod-enhancing glucose biosensor.The high affinity of chitosan for Au nanorods associated with its amino groups resulted in the formation of a layer of Au nanorods on the surface of Au electrode.It served as an intermediator to retain high efficient and stable immobilization of the enzyme.The performance of biosensors was investigated by cyclic voltammetry （CV）,in the presence of artificial redox mediator,ferrocenecarboxaldehyde.The biosensor had a fast response to glucose,and the response time was less than 10 s.The results indicated that the gold nanorods could enhance the current response to glucose.The detection limits of glucose can reach 10 mM,and the Michaelis-Menten constant K mapp is 13.62 mM.
LI Yingyinga,b,WANG Xiaoxiaa,DONG Xianga,HE Zhipenga,and ZHANG Haiqiana,c a College of Material Science and Technology,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China b Kunming Fire Service Training School,Kunming 650208,China c Jiangsu Laboratory for Biomaterials and Devices,Southeast University,Nanjing 210096,China
摘 要：Au nanorods were prepared by a seeding growth approach and used in fabricating the nanorod-enhancing glucose biosensor.The high affinity of chitosan for Au nanorods associated with its amino groups resulted in the formation of a layer of Au nanorods on the surface of Au electrode.It served as an intermediator to retain high efficient and stable immobilization of the enzyme.The performance of biosensors was investigated by cyclic voltammetry （CV）,in the presence of artificial redox mediator,ferrocenecarboxaldehyde.The biosensor had a fast response to glucose,and the response time was less than 10 s.The results indicated that the gold nanorods could enhance the current response to glucose.The detection limits of glucose can reach 10 mM,and the Michaelis-Menten constant K mapp is 13.62 mM.
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