Immobilization of RGD Peptidcs onto Decellularized Valve Scaffolds to Promote Cell Adhesion
来源期刊:Journal Of Wuhan University Of Technology Materials Science Edition2007年第4期
论文作者:史嘉玮
文章页码:686 - 690
摘 要:<正>Porcine aortic valves were decellularized with trypsinase/EDTA and Triton-100.With the help of a coupling reagent Sulfo-LC-SPDP,the biological valve scaffolds were immobilized with one of RGD (arginine-glycine-aspartic acid)containing peptides,called GRGDSPC peptide.Myofibroblasts harvested from rats were seeded onto them.Based on the spectra of X-ray photoelectron spectroscopy,we could find conjugation of GRGDSPC peptide and the scaffolds.Cell count by both microscopy and MTT assay showed that myofibroblasts were easier to adhere to the modified scaffolds.It is proved that it is feasible to immobilize RGD peptides onto decellularized valve scaffolds,and effective to promote cell adhesion,which is beneficial for constructing tissue engineering heart valves in vitro.
史嘉玮
摘 要:<正>Porcine aortic valves were decellularized with trypsinase/EDTA and Triton-100.With the help of a coupling reagent Sulfo-LC-SPDP,the biological valve scaffolds were immobilized with one of RGD (arginine-glycine-aspartic acid)containing peptides,called GRGDSPC peptide.Myofibroblasts harvested from rats were seeded onto them.Based on the spectra of X-ray photoelectron spectroscopy,we could find conjugation of GRGDSPC peptide and the scaffolds.Cell count by both microscopy and MTT assay showed that myofibroblasts were easier to adhere to the modified scaffolds.It is proved that it is feasible to immobilize RGD peptides onto decellularized valve scaffolds,and effective to promote cell adhesion,which is beneficial for constructing tissue engineering heart valves in vitro.
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