Dissociation of outer membrane for Escherichia coli cell caused by cerium nitrate
来源期刊：JOURNAL OF RARE EARTHS2010年第2期
论文作者：陈爱美 施庆珊 冯劲 欧阳友生 陈仪本 谭绍早
文章页码：312 - 315
摘 要：The biological effect of cerium nitrate on the outer membrane（OM） of Escherichia coli（E.coli） cell was studied,and the antim-icrobial mechanism of rare earth elements was explored.The antimicrobial effect of cerium nitrate on E.coli cell was valued by plate count method,and the morphology change of E.coli cell was observed with scanning electron microscopy（SEM） and transmission electron microscopy（TEM）.The results showed that the E.coli cell suspension was flocculated when the concentration of Ce（NO3）3?6H2O solution was greater than or equal to 100 mg/L,which may be due to the alteration of charge and hydrophobicity of the E.coli cell surface.The antimicro-bial assay suggested that 100 mg/L Ce（NO3）3?6H2O solution could kill 80.69% of E.coli cells,and the killing rate reached nearly 99% at 1000 mg/L.The antimicrobial target site of rare earth elements was observed on the cell wall in SEM and TEM images.With the concentration of Ce（NO3）3·6H2O solution increasing,the OM of E.coli cell was separated（≤100 mg/L）,dissociated（100～500 mg/L） or even departed（≥500 mg/L） from cytoplasmic membrane（CM）.
陈爱美1,2,3，施庆珊1,2,3，冯劲1,2,3，欧阳友生1,2,3，陈仪本1,2,3，谭绍早4
1. Guangdong Institute of Microbiology2. Guangdong Open Laboratory of Applied Microbiology3. Guangdong Province Key Laboratory of Microbial Culture Collection and Application4. Department of Chemistry,Jinan University
摘 要：The biological effect of cerium nitrate on the outer membrane（OM） of Escherichia coli（E.coli） cell was studied,and the antim-icrobial mechanism of rare earth elements was explored.The antimicrobial effect of cerium nitrate on E.coli cell was valued by plate count method,and the morphology change of E.coli cell was observed with scanning electron microscopy（SEM） and transmission electron microscopy（TEM）.The results showed that the E.coli cell suspension was flocculated when the concentration of Ce（NO3）3?6H2O solution was greater than or equal to 100 mg/L,which may be due to the alteration of charge and hydrophobicity of the E.coli cell surface.The antimicro-bial assay suggested that 100 mg/L Ce（NO3）3?6H2O solution could kill 80.69% of E.coli cells,and the killing rate reached nearly 99% at 1000 mg/L.The antimicrobial target site of rare earth elements was observed on the cell wall in SEM and TEM images.With the concentration of Ce（NO3）3·6H2O solution increasing,the OM of E.coli cell was separated（≤100 mg/L）,dissociated（100～500 mg/L） or even departed（≥500 mg/L） from cytoplasmic membrane（CM）.
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