Effect of cerium ion on the proliferation,differentiation and mineralization function of primary mouse osteoblasts in vitro
来源期刊：Journal of Rare Earths2010年第1期
论文作者：张金超 刘翠莲 李亚平 孙静 王鹏 邸科前 赵燕燕
文章页码：138 - 142
摘 要：The effects of cerium ion（Ce3+） on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary mouse osteoblasts（OBs） were investigated.The results indicated that Ce3+ at all concentrations（1×10-9,1×10-8,1×10-7,1×10-6,1×10-5,and 1×10-4 mol/L） promoted the proliferation of osteoblasts（OBs）.On day 1 and 3,Ce3+ promoted the differentiation of OBs at concentrations of 1×10-9,1×10-7,and 1×10-6 mol/L,but inhibited the differentiation of OBs at higher concentrations.On day 2,Ce3+ inhibited the differentiation of OBs at tested concentrations.On day 9 and 12,Ce3+ inhibited the adipocytic transdifferentiation of OBs at most concentrations.On day 15,Ce3+ promoted the adipocytic transdifferentiation of OBs at concentrations of 1×10-9,1×10-6,1×10-5,and 1×10-4 mol/L,but had no effects at other concentrations.Ce3+ inhibited the formation of mineralized matrix nodules of OBs at concentrations of 1×10-9,1×10-8 and 1×10-7 mol/L,and promoted the formation of mineralized matrix nodules of OBs at other concentrations.These findings suggested that the effects of Ce3+ on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary OBs depended on the concentration and culture time;moreover,they were pivotal factors for switching the biological effects of Ce3+ from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.
张金超1，刘翠莲1，李亚平1，孙静2，王鹏2，邸科前3，赵燕燕3
1. Chemical Biology Laboratory,College of Chemistry & Environmental Science,Hebei University2. Affiliated Hospital of Hebei University3. Experimental Center,Hebei University
摘 要：The effects of cerium ion（Ce3+） on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary mouse osteoblasts（OBs） were investigated.The results indicated that Ce3+ at all concentrations（1×10-9,1×10-8,1×10-7,1×10-6,1×10-5,and 1×10-4 mol/L） promoted the proliferation of osteoblasts（OBs）.On day 1 and 3,Ce3+ promoted the differentiation of OBs at concentrations of 1×10-9,1×10-7,and 1×10-6 mol/L,but inhibited the differentiation of OBs at higher concentrations.On day 2,Ce3+ inhibited the differentiation of OBs at tested concentrations.On day 9 and 12,Ce3+ inhibited the adipocytic transdifferentiation of OBs at most concentrations.On day 15,Ce3+ promoted the adipocytic transdifferentiation of OBs at concentrations of 1×10-9,1×10-6,1×10-5,and 1×10-4 mol/L,but had no effects at other concentrations.Ce3+ inhibited the formation of mineralized matrix nodules of OBs at concentrations of 1×10-9,1×10-8 and 1×10-7 mol/L,and promoted the formation of mineralized matrix nodules of OBs at other concentrations.These findings suggested that the effects of Ce3+ on the proliferation,differentiation,adipocytic transdifferentiation and mineralization function of primary OBs depended on the concentration and culture time;moreover,they were pivotal factors for switching the biological effects of Ce3+ from toxicity to activity,from damage to protection,or from down-regulation to up-regulation.
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