Design and Fabrication of Superparamaganitic Hybrid Microspheres for Protein Immobilization
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2011年第6期
论文作者：任琦 陈明清
文章页码：1084 - 1088
摘 要：Superparamagnetic poly（styrene）-co-poly（2-acrylanmido-2-methyl propanesulfonic acid） （PSt-co-PAMPS） and poly（methylmethacrylate）-co-poly（glycidyl methacrylate） （PMMA-co-PGMA） microspheres with mean size of 170 nm were prepared by emulsion polymerization in the presence of oleic acid-coated Fe3O4 nanoparticles. The structures, morphologies, diameter and diameter distribution of the as-prepared microspheres were identified by Fourier transform infrared spectroscopy （FT-IR） and transmission electron microscopy （TEM）. The saturation magnetizations of PSt-co-PAMPS and PMMA-co-PGMA microspheres are 21.94 and 25.07 emu/g, respectively. The as-synthesized magnetic microspheres were used for immobilization of Bovine serum albumin （BSA） by physical interaction and covalent interaction respectively. The equilibrium amount of BSA immobilized onto PMMA-co-PGMA microspheres was 86.48 mg/g microspheres in 90 min, while on PSt-co-PAMPS microspheres was 59.62 mg/g microspheres in 120 min.
任琦，陈明清
School of Chemical and Material Engineering,Jiangnan University
摘 要：Superparamagnetic poly（styrene）-co-poly（2-acrylanmido-2-methyl propanesulfonic acid） （PSt-co-PAMPS） and poly（methylmethacrylate）-co-poly（glycidyl methacrylate） （PMMA-co-PGMA） microspheres with mean size of 170 nm were prepared by emulsion polymerization in the presence of oleic acid-coated Fe3O4 nanoparticles. The structures, morphologies, diameter and diameter distribution of the as-prepared microspheres were identified by Fourier transform infrared spectroscopy （FT-IR） and transmission electron microscopy （TEM）. The saturation magnetizations of PSt-co-PAMPS and PMMA-co-PGMA microspheres are 21.94 and 25.07 emu/g, respectively. The as-synthesized magnetic microspheres were used for immobilization of Bovine serum albumin （BSA） by physical interaction and covalent interaction respectively. The equilibrium amount of BSA immobilized onto PMMA-co-PGMA microspheres was 86.48 mg/g microspheres in 90 min, while on PSt-co-PAMPS microspheres was 59.62 mg/g microspheres in 120 min.
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