SYNTHESIS AND PURIFICATION OF MODIFIED ANTISENSE OLIGODEOXYRIBONUCLEOTIDES
来源期刊:中南大学学报(英文版)1997年第1期
论文作者:Li Xiaoru Wen Zhibin
文章页码:49 - 53
Key words:oligodeoxyribonucleotedes;synthesis;purification;high performance liquid chromatography
Abstract: The synthesis and purification of modified antisense of igodeoxyriboucleotides (MAO ) are des cribed. A new oligonucleotide purification method and a novel activating agent, dimethyl sulfoxide, for thecondensation reaction are used. EDTA has been attached covalently to the 5' end of the oligodeoxyribonucleotides sequence via an ethylenediamine linker in basic triethylammonium solution, then chelated withthe metal ions such as Fe(Ⅱ), Co(Ⅱ) or Cu(Ⅱ). High performance liquid chromatorgaphy is used to separate and purify this kind of MAO, using double mobile phase, triethylammonium acetate and acetonitrile. Byusing this routine chemical synthesis method, EDTA can be linked to the 5' terminus of oligodeoxyribonucleotedes of any desired length and sequence, forming chimeric MAOs.