Effects of lanthanum on POD expression and DNA methylation of purple pepper under salt stress
来源期刊：Journal of Rare Earths2014年第5期
论文作者：胡能兵 隋益虎 蔡永萍 樊洪泓 林毅
文章页码：467 - 475
摘 要：Pepper seedlings were hydroponically cultivated in 0–20 mg/L extraneous lanthanum chloride（La3+） for different numbers of days to investigate its regulating effects under salt stress. The results showed that, depending on the salt stress of 0.75 g/L, a lower concentration of La3+（1.0 mg/L） might result in the lowest death rate, increase POD activity and change the band type of the stem and root on the 8th day. Furthermore, a methylation analysis by the methylation-sensitive amplified polymorphism（MSAP） technique indicated that a lower concentration of La3+（1.0 mg/L） triggered the lowest methylation level of 26.1%, of which the hypermethylation events were the primary type of methylation. In addition, the sequencing of 14 differentially expressed polymorphic fragments and the subsequent blast search revealed that La3+ could induce methylation events in salt-tolerance sequences.
胡能兵1,2，隋益虎2，蔡永萍1，樊洪泓1，林毅1
1. Department of Life Science, Anhui Agricultural University2. Department of Plant and Science,Anhui Science and Technology University
摘 要：Pepper seedlings were hydroponically cultivated in 0–20 mg/L extraneous lanthanum chloride（La3+） for different numbers of days to investigate its regulating effects under salt stress. The results showed that, depending on the salt stress of 0.75 g/L, a lower concentration of La3+（1.0 mg/L） might result in the lowest death rate, increase POD activity and change the band type of the stem and root on the 8th day. Furthermore, a methylation analysis by the methylation-sensitive amplified polymorphism（MSAP） technique indicated that a lower concentration of La3+（1.0 mg/L） triggered the lowest methylation level of 26.1%, of which the hypermethylation events were the primary type of methylation. In addition, the sequencing of 14 differentially expressed polymorphic fragments and the subsequent blast search revealed that La3+ could induce methylation events in salt-tolerance sequences.
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