Preparation and Characterization of PEGDE Crosslinked Silk Fibroin Film
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2014年第5期
论文作者：魏雅丽 SUN Dan YI Honggen ZHAO Huanrong 王建南
文章页码：1083 - 1089
摘 要：To obtain water-insoluble silk fibroin（SF） materials, polyethylene glycol diglycidyl ether（PEG-DE） was selected as a crosslinking agent to prepare SF films（blends）. The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF:PEG-DE was 1.0:0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form β-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend films. In order to demonstrate the cytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend film（SF:PEG-DE = 1:1） and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend film resulting in high cell viability. The extracted liquid from the SF porous film did not inhibit cell proliferation, as estimated by the MTT assay.
魏雅丽，SUN Dan，YI Honggen，ZHAO Huanrong，王建南
National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University
摘 要：To obtain water-insoluble silk fibroin（SF） materials, polyethylene glycol diglycidyl ether（PEG-DE） was selected as a crosslinking agent to prepare SF films（blends）. The reaction conditions were optimized for the crosslinking of the SF molecules. The hot water stability of the blends was measured using BCA protein assay and gravimetric analysis. The molecular conformation and crystalline structure of the blends were analyzed by FTIR and XRD, respectively. When the mass ratio of SF:PEG-DE was 1.0:0.8, the hot water loss rate of the SF blends was minimized. PEG-DE could induce SF molecules to form β-sheets during the gel reaction process, resulting in improved crystallinity and hot water dissolved resistance of the blend films. In order to demonstrate the cytotoxicity of the chemical reagents used to crosslink SF, L929 cells were seeded on the blend film（SF:PEG-DE = 1:1） and cultured for 3 days. Cells of L929 readily adhered and spread in the fusiform on the blend film resulting in high cell viability. The extracted liquid from the SF porous film did not inhibit cell proliferation, as estimated by the MTT assay.
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