Chemical fingerprinting of Su-He-Xiang-Wan and attribution of major characteristic peaks for its quality control by GC-MS
来源期刊：中南大学学报(英文版)2013年第8期
论文作者：WANG Wei-ping(王伟萍) LIN Juan(蔺娟) ZHANG Liang-xiao(张良晓) ZHANG Ming-yue(张明玥) LIANG Yi-zeng(梁逸曾)
文章页码：2115 - 2123
Key words：chromatographic fingerprint; attribution analysis; quality control; herbal medicines; complex prescription
Abstract: A simple and facile gas chromatography-mass spectrometer (GC-MS) fingerprint of Su-He-Xiang-Wan (SHXW) was developed, the similarity analysis was conducted, and attribution of the major characteristic peaks was identified for SHXW quality control. GC-MS analysis was performed on a QP2010 instrument (Shimadzu, Japan) equipped with a capillary column of RTX-5MS. The column temperature was initiated at 50 °C, held for 5 min, increased at the rate of 3 °C/min to 120 °C, held for 2 min, and then increased at the rate of 4 °C/min to 220 °C, held for 10 min. Helium carrier gas was used at a constant flow rate of 1.3 mL/min. Mass conditions were ionization voltage, 70 eV; injector temperature, 250 °C; ion source temperature, 250 °C; splitting ratio, 30:1; full scan mode in the 40-500 Da mass ranges with rate of 0.2 s per scan. Attribution of the major characteristic peaks was identified for SHXW by comparing the chemical standards, references of Chinese herbal medicines and the negative controls of prescription samples (NC) of SHXW. With the help of the temperature-programmed retention indices (PTRIs) used together with mass spectra and chemical standards, 25 major characteristic peaks have been identified. Nine volatile medicinal materials were identified in the prescription of SHXW by attributing to the 27 major characteristic peaks. The results demonstrate that the proposed method is a powerful approach to quality control of complex herbal medicines.
WANG Wei-ping(王伟萍)1, 2, LIN Juan(蔺娟)2, ZHANG Liang-xiao(张良晓)3, ZHANG Ming-yue(张明玥)2, LIANG Yi-zeng(梁逸曾)1
(1. Research Center of Modernization of Chinese Herbal Medicine, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
2. Xinjiang Uygur Autonomous Region Institute for Food and Drug Control, Urumqi 830004, China;
3. Oilcrops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)
Abstract:A simple and facile gas chromatography-mass spectrometer (GC-MS) fingerprint of Su-He-Xiang-Wan (SHXW) was developed, the similarity analysis was conducted, and attribution of the major characteristic peaks was identified for SHXW quality control. GC-MS analysis was performed on a QP2010 instrument (Shimadzu, Japan) equipped with a capillary column of RTX-5MS. The column temperature was initiated at 50 °C, held for 5 min, increased at the rate of 3 °C/min to 120 °C, held for 2 min, and then increased at the rate of 4 °C/min to 220 °C, held for 10 min. Helium carrier gas was used at a constant flow rate of 1.3 mL/min. Mass conditions were ionization voltage, 70 eV; injector temperature, 250 °C; ion source temperature, 250 °C; splitting ratio, 30:1; full scan mode in the 40-500 Da mass ranges with rate of 0.2 s per scan. Attribution of the major characteristic peaks was identified for SHXW by comparing the chemical standards, references of Chinese herbal medicines and the negative controls of prescription samples (NC) of SHXW. With the help of the temperature-programmed retention indices (PTRIs) used together with mass spectra and chemical standards, 25 major characteristic peaks have been identified. Nine volatile medicinal materials were identified in the prescription of SHXW by attributing to the 27 major characteristic peaks. The results demonstrate that the proposed method is a powerful approach to quality control of complex herbal medicines.
Key words:chromatographic fingerprint; attribution analysis; quality control; herbal medicines; complex prescription
