Characterization of a newly Isolated Bacterium Pandoraea sp. B-6 capable of degrading kraft Lignin
来源期刊：中南大学学报(英文版)2013年第3期
论文作者：ZHENG Yu(郑玉) CHAI Li-yuan(柴立元) YANG Zhi-hui(杨志辉) ZHANG Huan(张欢) CHEN Yue-hui(陈跃辉)
文章页码：757 - 763
Key words：kraft lignin; Pandoraea sp.; biodegradation; gas chromatography-mass spectrophotometry; relative molecular mass distribution
Abstract: A newly isolated bacterium was screened out for its survival on medium with 6 000 mg/L kraft lignin as the sole carbon source and energy, and identified as Pandoraea sp. by 16S rRNA gene sequence analysis. The biodegradation experiment was carried out in mineral salt medium, containing 5 000 mg/L kraft lignin as only carbon and energy at pH 8.0 and 30 °C. Under these conditions, significant reduction in color and lignin content by the strain was observed after incubation for 5 d. The strain attains maximum reduction capability in color (44.6%) and lignin content (39.9%) within 5 d of incubation, and reduced chemical oxygen demand(COD) from initial concentration 7 399 to 3 980 mg/L at maximum reduction level of 46.2% on the 4th day. The total ion chromatograph (TIC) of compounds presented in the chloroform extract of control and bacterial treated samples shows the formation of several lignin-related aromatic compounds including some small molecular lignin fragments, indicating a strong destruction in the lignin structure.
ZHENG Yu(郑玉)1, CHAI Li-yuan(柴立元)1,2, YANG Zhi-hui(杨志辉)1,2, ZHANG Huan(张欢)1, CHEN Yue-hui(陈跃辉)1
(1. Institute of Environmental Science & Engineering, School of Metallurgical Science and Engineering,
Central South University, Changsha 410083, China;
2. Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution,
Changsha 410083, China)
Abstract:A newly isolated bacterium was screened out for its survival on medium with 6 000 mg/L kraft lignin as the sole carbon source and energy, and identified as Pandoraea sp. by 16S rRNA gene sequence analysis. The biodegradation experiment was carried out in mineral salt medium, containing 5 000 mg/L kraft lignin as only carbon and energy at pH 8.0 and 30 °C. Under these conditions, significant reduction in color and lignin content by the strain was observed after incubation for 5 d. The strain attains maximum reduction capability in color (44.6%) and lignin content (39.9%) within 5 d of incubation, and reduced chemical oxygen demand(COD) from initial concentration 7 399 to 3 980 mg/L at maximum reduction level of 46.2% on the 4th day. The total ion chromatograph (TIC) of compounds presented in the chloroform extract of control and bacterial treated samples shows the formation of several lignin-related aromatic compounds including some small molecular lignin fragments, indicating a strong destruction in the lignin structure.
Key words:kraft lignin; Pandoraea sp.; biodegradation; gas chromatography-mass spectrophotometry; relative molecular mass distribution
