Biochemical effects of gadolinium chloride in rats liver and kidney studied by ~1H NMR metabolomics
来源期刊：Journal of Rare Earths2009年第2期
论文作者：廖沛球 魏来 吴惠丰 李伟生 吴亦洁 李晓晶 倪嘉缵 裴奉奎
文章页码：280 - 287
摘 要：The biochemical effects of gadolinium chloride were studied using high-resolution 1H nuclear magnetic resonance （NMR） spectroscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous extracts obtained from control and gadolinium chloride （GdCl3） （10 and 50 mg/kg body weight, intraperitoneal injection, i.p.） treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCl3, and extracted using methanol/chloroform solvent system. 1H NMR spectra of tissue extracts were analyzed by pattern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and decreased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine （GPC）, betaine, myo-inositol and trimethylamine N-oxide （TMAO） levels in kidney illustrated kidney disturbance induced by GdCl3.
廖沛球1,2，魏来1,2，吴惠丰1，李伟生1,2，吴亦洁1，李晓晶1，倪嘉缵1，裴奉奎1
1. Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China2. Graduate School of the Chinese Academy of Sciences,Beijing 100039,China
摘 要：The biochemical effects of gadolinium chloride were studied using high-resolution 1H nuclear magnetic resonance （NMR） spectroscopy to investigate the biochemical composition of tissue （liver and kidney） aqueous extracts obtained from control and gadolinium chloride （GdCl3） （10 and 50 mg/kg body weight, intraperitoneal injection, i.p.） treated rats. Tissue samples were collected at 48, 96 and 168 h p.d. after exposure to GdCl3, and extracted using methanol/chloroform solvent system. 1H NMR spectra of tissue extracts were analyzed by pattern recognition using principal components analysis. The liver damages caused by GdCl3 were characterized by increased succinate and decreased glycogen level and elevated lactate, alanine and betaine concentration in liver. Furthermore, the increase of creatine and lactate, and decrease of glutamate, alanine, phosphocholine, glycophosphocholine （GPC）, betaine, myo-inositol and trimethylamine N-oxide （TMAO） levels in kidney illustrated kidney disturbance induced by GdCl3.
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