Preparation and Characterization of PD LIA/Chondroitin Sulfate/Chitosan Seaffold for Peripheral Nerve Regeneration

XU Haixing~(1,2) YAN Yuhua~1 WAN Tao~1 LI Shipu~1 (1.Biomedical Materials and Engineering Research Center,Wuhan University of Technology,Wuhan 430070,China; 2.Department of Pharmaceutical Engineering,School of Chemical Engineering,Wuhan University of Technology,Wuhan 430070,China)

摘 要：<正>A novel bioactive and bioresorbable PDLLA/chondroitin sulfate/chitosan scaffold was prepared via layer-by-layer（LBL）electrostatic-self-assembly（ESA）and the thermally induced phase separation （TIPS）technique.Chondroitin sulfate and chitosan were alternately deposited on the activated PDLLA substrate. The deposition process was monitored by UV-Vis absorbance spectroscopy.After frozen and lyophilized,the scaffold was characterized by attenuated total reflection（ATR）-FT-IR,XPS,SEM and AFM.The results showed that the scaffold was modified uniformly with a dehse inner layer with few detectable pores and a porous sponge outer layer with the pore size about 5μm,there was an obvious across section and the average thickness of each layer was about 9.4 nm.

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