Upconversion nanoparticles for differential imaging of plant cells and detection of fluorescent dyes
来源期刊：Journal of Rare Earths2016年第2期
论文作者：吴笑峰 胡盼 胡仕刚 陈增辉 严焕元 唐志军 席在芳 余意 戴港涛 刘云新
文章页码：208 - 220
摘 要：Upconversion NaLuF4 nanoparticles were synthesized by the solvothermal method which could emit multicolor visible light under the excitation of 980 nm near-infrared（NIR） photons. These upconversion nanoparticles（UCNPs） with an acidic ligand could rapidly capture the basic rhodamine-B（RB） in plant cells to generate a close UCNPs@RB system. RB could efficiently absorb the green fluorescence from NaLuF4:18 mol.%Yb3+,2 mol.%Er3+ UCNPs and then emitted red light in the UCNPs@RB system by a robust luminescence resonance energy transfer（LRET） from UCNPs to RB. The detection limit of RB with these upconversion fluorescent nanoprobes could reach 0.25 μg/cm3 in plant cell even under an ultra low excitation power source of 0.2 W/mm2. This LRET phenomenon was also extended to Na Lu F4:18 mol.%Yb3+,0.5 mol.%Tm3+@Sodium fluorescein（SF） system. In addition, the differential imaging could be achieved by successively incubating plant cells with fluorescent dyes and UCNPs. The fluorescent dyes aggregated in cell wall while UCNPs with surface modification distributed both in cell wall and cytoplasm, so that UCNPs@Dyes formed in cell walls which could emit multicolor light by LRET which was different from the emission in cytoplasm with only UCNPs.
吴笑峰1，胡盼1，胡仕刚1，陈增辉2，严焕元3，唐志军1，席在芳1，余意1，戴港涛2，刘云新2,4
1. School of Information and Electrical Engineering,Hunan University of Science and Technology2. Department of Physics and Electrical Science,Hunan University of Science and Technology3. College of Mechanical and Electrical Engineering,Hunan University of Science and Technology
摘 要：Upconversion NaLuF4 nanoparticles were synthesized by the solvothermal method which could emit multicolor visible light under the excitation of 980 nm near-infrared（NIR） photons. These upconversion nanoparticles（UCNPs） with an acidic ligand could rapidly capture the basic rhodamine-B（RB） in plant cells to generate a close UCNPs@RB system. RB could efficiently absorb the green fluorescence from NaLuF4:18 mol.%Yb3+,2 mol.%Er3+ UCNPs and then emitted red light in the UCNPs@RB system by a robust luminescence resonance energy transfer（LRET） from UCNPs to RB. The detection limit of RB with these upconversion fluorescent nanoprobes could reach 0.25 μg/cm3 in plant cell even under an ultra low excitation power source of 0.2 W/mm2. This LRET phenomenon was also extended to Na Lu F4:18 mol.%Yb3+,0.5 mol.%Tm3+@Sodium fluorescein（SF） system. In addition, the differential imaging could be achieved by successively incubating plant cells with fluorescent dyes and UCNPs. The fluorescent dyes aggregated in cell wall while UCNPs with surface modification distributed both in cell wall and cytoplasm, so that UCNPs@Dyes formed in cell walls which could emit multicolor light by LRET which was different from the emission in cytoplasm with only UCNPs.
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