Effects of cerous nitrate on growth and tanshinone production in salvia miltiorrhiza hairy roots
来源期刊：Journal of Rare Earths2015年第11期
论文作者：韩名宇 郭万里 梁宗锁 杨东风 闫希军 朱永宏 刘岩
文章页码：1228 - 1235
摘 要：Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce3+ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I（DTI） and cryptotanshinone（CT） were significantly enhanced by 50 μmol/L Ce3+, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A（TIIA） and tanshinone I（TI） contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes（HMGR, DXR, DXS1, DXS2 and GGPPS） involved in tanshinone biosynthesis were upregulated by Ce3+. Responses of DXS1, DXS2 and GGPPS to Ce3+ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.
韩名宇1,2，郭万里1,2，梁宗锁1,2，杨东风1,2，闫希军3，朱永宏3，刘岩3
1. College of Life Science, Zhejiang Sci-Tech University2. Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province3. Tasly R&D Institute, Tasly Holding Group Co., Ltd.
摘 要：Salvia miltiorrhiza, a popular traditional Chinese medicine, is widely used for treatments in cardiotonic disease. Tanshinones are a group of bioactive ingredients in S. miltiorrhiza. In this study, Ce3+ was used as an elicitor to enhance tanshinones production in S. miltiorrhiza hairy roots. The results showed that contents of dihydrotanshinone I（DTI） and cryptotanshinone（CT） were significantly enhanced by 50 μmol/L Ce3+, and reached to 0.875 mg/g and 0.271 mg/g, respectively. However, tanshinone II A（TIIA） and tanshinone I（TI） contents were significantly decreased to 59% and 62% of the control. Simultaneously, expressions of genes（HMGR, DXR, DXS1, DXS2 and GGPPS） involved in tanshinone biosynthesis were upregulated by Ce3+. Responses of DXS1, DXS2 and GGPPS to Ce3+ treatments were later than HMGR and DXR. We speculated that branch pathways of DTI and CT biosynthesis were probably different from TIIA and TI. This work will help us understand biosynthetic mechanism of tanshinones in plants.
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