Biocompatibility evaluation in vitro. Part III: Cytotoxicity expression of human and animal osteoblasts on the biomaterials

来源期刊：中南大学学报(英文版)2001年第3期

论文作者：RUAN Jian-ming M.H. Grant

文章页码：147 - 155

Key words：biomaterial; cytotoxicity; biological molecular technique

Abstract: Exclusion and inflammation in the clinic are observed for various reasons including material chemical composition, physical properties aswell as macro-and micro-structure of the implants, surface condition of the implants, and also patient dependent factors. Cytotoxicity expression of cells is a central issue in current biocompatibility to screen the potential implantmaterials and drugs. This studywas aimed at investigation reaction between the potential implantmaterials and surround tissue. Cytotoxicityof human and ratosteoblast in the material extracts was determinated by testing standards such as GHS assay, MTT assay, alkaline phosphatase activity assay, LDH assay, and Lowry assay. Research results demonstrated that compared with the control condition polystyrene culture plate both human and rat osteoblast cells have normal phenotypic expression in hydroxyapatite extract, and this expression was statistically restricted in hydroxyapatite-spinel extract. However, this restrict, e.g. cytotoxicity could be partially eliminated by immersion treatment of the materials in culture medium.