虎杖中白藜芦醇的提取工艺

来源期刊：中南大学学报(自然科学版)2004年第6期

论文作者：向海艳 周春山 钟世安 陈龙胜

文章页码：965 - 969

关键词：虎杖;白藜芦醇;提取工艺

Key words：Polygonum Cuspidatum Sieb et Zucc; resveratrol; extraction process

摘    要：采用HPLC法,以白藜芦醇的含量为指标,通过单因素实验及正交实验方法确定了从虎杖中提取白藜芦醇的最佳工艺条件。HPLC法的色谱分析条件为:YWG-ODS色谱柱(长×直径为250 mm×4.6 mm,粒径为5μm),以φ_甲醇∶φ_水=45∶55为流动相,流速为0.5 mL/min,检测波长为303 nm。HPLC法测定结果表明,以体积分数为80%的乙醇水溶液为提取剂,液固比为14∶1,在50℃下提取2次,每次提取2 h,从虎杖中提取的白藜芦醇质量分数可达0.301%。 关键词:虎杖;白藜芦醇;提取工艺

Abstract: The extract conditions of resveratrol from Polygonum Cuspidatum Sieb et Zucc were investigated by using the orthogonal design with resveratrol as index,whichwas determined byHPLC.HPLC detemination conditions were:YWG column(250 mm×4.6 mm, diameter 5μm), with the mobile phase of 45% CH3OH(ratio of volume), the flow rate of 0.5 mL/min and the detection wave length of 303 nm.The results show that the optimum extract conditions of resveratrol from Polygonum Cuspidatum Sieb et Zucc are that, with 14 times amount of 80%(ratio of volume) alcohol under 50℃, the extraction can be done twice, two hours each time.HPLC determination shows that under this extract conditions ,the content of resveratrol in PolygonumCuspidatum Sieb et Zucc is 0.301 % approximately.