Biocompatibility of Surface-Modified Biphasic Calcium Phosphate/Poly-L-Lactide Biocomposite in vitro and in vivo

Weizhong Yang1), Guangfu Yin1), Dali Zhou1), Jianwen Gu2), Yadong Li2) and Hujun Zhang2) 1) College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China 2) Chengdu Military General Hospital, Chengdu 610083, China

Õª Òª£ºThe biocompatibility of surface-modified biphasic calcium phosphate £¨mBCP£©/poly-L-Lactide £¨PLLA£© biocomposite was investigated through a series of experiments in vitro and in vivo. Acute toxicity and short term systemic toxicity experiments revealed no toxicity of the materials. Hemolysis assay indicated the good blood compatibility of the composite. In cytotoxicity assay, L929 mouse fibroblasts could well differentiate and proliferate. Animal experiments in vivo were performed by implanting the materials into rabbits muscle for 8 weeks. The decreasing of inflammatory cells, the building of fibrous tissue layer as well as the growing of blood cells into materials indicated the nontoxicity of the composite. Based on the experiment results, surfacemodified BCP/PLLA biocomposite is proven to have superior biocompatibility, which would be a promising bone repairing material.

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