Injectable silk/hydroxyapatite nanocomposite hydrogels with vascularization capacity for bone regeneration
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2021年第4期
论文作者：Keke Wang Weinan Cheng Zhaozhao Ding Gang Xu Xin Zheng Meirong Li Guozhong Lu Qiang Lu
摘 要：Localized and sustained osteogenic-angiogenic stimulation to bone defects is critical for effective bone repair. Here, desferrioxamine（DFO） was loaded on silk fibroin nanofibers and blended with hydroxyapatite nanorods（HA）, forming injectable DFO-loaded silk fibroin-HA nanocomposite hydrogels. The composite hydrogels remained homogeneous distribution of HA with high ratio（60 %） and also higher stiffness than that of pure silk fibroin nanofiber hydrogels, which provided stable osteogenic stimulation niches for tissue regeneration. Without the scarify of injectability, the hydrogels achieved slow delivery of DFO for above 60 days, resulting in suitable angiogenesis in vitro and in vivo and better osteogenesis than DFO-free systems. Compared to previous injectable silk fibroin-HA hydrogels, the introduction of vascularization capacity further stimulated the osteogenic differentiation of stem cells and accelerated new bone formation. Quicker and better bone healing were detected at defect sites after the injection of DFO-loaded nanocomposite hydrogels, indicating the effective synergistic effect of osteogenic and angiogenic cues. This work provides a simple and effective strategy of introducing angiogenic cues to bone matrices. We believe that the injectable nanocomposite hydrogels are suitable for the regeneration of bone tissues.
Keke Wang1,2，Weinan Cheng3，Zhaozhao Ding2，Gang Xu4，Xin Zheng4，Meirong Li5，Guozhong Lu2，Qiang Lu2
1. College of Chemistry, Chemical Engineering and Materials Science & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University2. Department of Burns and Plastic Surgery, The Affiliated Hospital of Jiangnan University3. Department of Orthopedics, The First Affiliated Hospital of Xiamen University4. Department of Orthopedics, The Second Affiliated Hospital of Soochow University5. Institute of Basic Medical Science, Wound Healing and Cell Biology Laboratory, Chinese PLA General Hospital
摘 要：Localized and sustained osteogenic-angiogenic stimulation to bone defects is critical for effective bone repair. Here, desferrioxamine（DFO） was loaded on silk fibroin nanofibers and blended with hydroxyapatite nanorods（HA）, forming injectable DFO-loaded silk fibroin-HA nanocomposite hydrogels. The composite hydrogels remained homogeneous distribution of HA with high ratio（60 %） and also higher stiffness than that of pure silk fibroin nanofiber hydrogels, which provided stable osteogenic stimulation niches for tissue regeneration. Without the scarify of injectability, the hydrogels achieved slow delivery of DFO for above 60 days, resulting in suitable angiogenesis in vitro and in vivo and better osteogenesis than DFO-free systems. Compared to previous injectable silk fibroin-HA hydrogels, the introduction of vascularization capacity further stimulated the osteogenic differentiation of stem cells and accelerated new bone formation. Quicker and better bone healing were detected at defect sites after the injection of DFO-loaded nanocomposite hydrogels, indicating the effective synergistic effect of osteogenic and angiogenic cues. This work provides a simple and effective strategy of introducing angiogenic cues to bone matrices. We believe that the injectable nanocomposite hydrogels are suitable for the regeneration of bone tissues.
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