Effects of Rare Earth Ions on NMR Spectrum of Anticoagulation Factor from Snake Venom
来源期刊：JOURNAL OF RARE EARTHS2001年第1期
论文作者：刘清亮 吴双顶 徐小龙 王守业
Abstract: The effects of Pr3+, Gd3+ ions on the 1H NMR spectrum of anticoagulation factor (ACF) from snake venom were investigated. It was observed that the quartet peaks at δ 4.16 and the triplet peaks at δ 1.37 are gradually broadened with the increase of Pr3+ ion content, but the broadening effects of Pr3+ ion on the two single peaks at δ 1.99 and δ 2.29 were not observed obviously, and the obvious chemical shifts of all peaks induced by Pr3+ were not found. Two peaks contributed to αH and βH of the Ala side chain in ACF are broadened by Gd3+ ion, while two single peaks at δ 1.99 and δ 2.29 are not affected basically by Gd3+ ion. This result proves that the distance between the Ca2+-binding site in ACF and αH or βH of the Ala side chain in ACF is shorter than that between the Ca2+-binding site in ACF and proton in Met residue.
刘清亮1，吴双顶1，徐小龙1，王守业1
(1.Department of Chemistry, University of Science and Technology of China,)
Abstract:The effects of Pr3+, Gd3+ ions on the 1H NMR spectrum of anticoagulation factor (ACF) from snake venom were investigated. It was observed that the quartet peaks at δ 4.16 and the triplet peaks at δ 1.37 are gradually broadened with the increase of Pr3+ ion content, but the broadening effects of Pr3+ ion on the two single peaks at δ 1.99 and δ 2.29 were not observed obviously, and the obvious chemical shifts of all peaks induced by Pr3+ were not found. Two peaks contributed to αH and βH of the Ala side chain in ACF are broadened by Gd3+ ion, while two single peaks at δ 1.99 and δ 2.29 are not affected basically by Gd3+ ion. This result proves that the distance between the Ca2+-binding site in ACF and αH or βH of the Ala side chain in ACF is shorter than that between the Ca2+-binding site in ACF and proton in Met residue.
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