Angiogenesis Induced by IKVAV-Containing Amphiphile Peptide in vivo
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2010年第5期
论文作者：宋玉林
文章页码：803 - 806
摘 要：An IKVAV （Isoleucine-Lysine-Valine-Alanine-Valine）-containing peptide amphiphile molecule （IKVAV-PA） was implanted subcutaneouly into rat backbone in the middle. Angiogenesis induced by IKVAV-PA was evaluated in vivo. 200 μL of 10, 2, 1 and 0.5 wt% IKVAV-PA solution were added into DMEM/F12 and self-assembled into nanofiber hydrogel. 1 mL of 1% IKVAV-PA （Experimental Group, EG） and 1 mL of 16.67% gelatin （Control Group, CG） were injected subcutaneously into rat backbone. The specimens were harvested two weeks after injection and examined immunohistochemically for VEGF（Vascular Endothelial Growth Factor）. TEM observations of hydrogels revealed a network of nanofibers, and there was a significant positive correlation between IKVAV-PA concentration and nanofiber alignment. Light microscopy observation showed capillary vessel with complete walls formed in hydrogel, with erythrocytes noted inside the vessels in EG; capillary vessels or erythrocytes were not found within gelatin in CG. Immunohistochemical analysis revealed that there were VEGF-positive cells within hydrogel, which were not found in CG. Self-assembled hydrogel from IKVAV-PA was able to induce the angiogenesis in vivo.
宋玉林
Department of Orthopaedics, the Second Affiliated Hospital of Nanchang University
摘 要：An IKVAV （Isoleucine-Lysine-Valine-Alanine-Valine）-containing peptide amphiphile molecule （IKVAV-PA） was implanted subcutaneouly into rat backbone in the middle. Angiogenesis induced by IKVAV-PA was evaluated in vivo. 200 μL of 10, 2, 1 and 0.5 wt% IKVAV-PA solution were added into DMEM/F12 and self-assembled into nanofiber hydrogel. 1 mL of 1% IKVAV-PA （Experimental Group, EG） and 1 mL of 16.67% gelatin （Control Group, CG） were injected subcutaneously into rat backbone. The specimens were harvested two weeks after injection and examined immunohistochemically for VEGF（Vascular Endothelial Growth Factor）. TEM observations of hydrogels revealed a network of nanofibers, and there was a significant positive correlation between IKVAV-PA concentration and nanofiber alignment. Light microscopy observation showed capillary vessel with complete walls formed in hydrogel, with erythrocytes noted inside the vessels in EG; capillary vessels or erythrocytes were not found within gelatin in CG. Immunohistochemical analysis revealed that there were VEGF-positive cells within hydrogel, which were not found in CG. Self-assembled hydrogel from IKVAV-PA was able to induce the angiogenesis in vivo.
关键词：
