Biodegradation of ferulic acid by a newly isolated strain of Cupriavidus sp. B-8
来源期刊：中南大学学报(英文版)2013年第7期
论文作者：CHAI Li-yuan(柴立元) ZHANG Huan(张欢) YANG Wei-chun(杨卫春) ZHU Yong-hua(朱咏华) YANG Zhi-hui(杨志辉) ZHENG Yu(郑玉) CHEN Yue-hui(陈跃辉)
文章页码：1964 - 1970
Key words：Cupriavidus sp. B-8; lignin biodegradation; ferulic acid; gas chromatography and mass spectrometry
Abstract: As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography (HPLC) analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.
CHAI Li-yuan(柴立元)1, ZHANG Huan(张欢)1, YANG Wei-chun(杨卫春)1, ZHU Yong-hua(朱咏华)2, YANG Zhi-hui(杨志辉)1, ZHENG Yu(郑玉)1, CHEN Yue-hui(陈跃辉)1
(1. National Engineering Research Center for Pollution Control of Heavy Metal,
(Institute of Environmental Science and Engineering, School of Metallurgical Science and Engineering,
Central South University), Changsha 410083, China;
2. State Key Laboratory of Chemo/Biosensing and Chemometrics (College of Biology, Hunan University),
Changsha 410082, China)
Abstract:As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography (HPLC) analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.
Key words:Cupriavidus sp. B-8; lignin biodegradation; ferulic acid; gas chromatography and mass spectrometry
