Cellular response to titanium discs coated with polyelectrolyte multilayer films

Jing Zhan¹，Qiao-jie Luo²，Ying Huang²，Xiao-dong Li²

1. Department of Stomatology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University2. Department of Oral and Maxillofacial Surgery, the Affiliated Stomatology Hospital, College of Medicine, Zhejiang University

摘 要：The purpose of this study was to investigate the effects of polyelectrolyte multilayer（PEM） coatings on the biological behavior of titanium（Ti） substrates. Collagen type Ι/hyaluronic acid（Col/HA） and chitosan/hyaluronic acid（Chi/HA） multilayer PEM coatings were introduced onto Ti substrates using layer-by-layer assembly. Contact angle instruments and quartz crystal microbalance were used for film characterization. The results obtained showed that both Col/HA and Chi/HA surfaces had high hydrophilicity and promoted cell adhesion in MC3T3-E1 pre-osteoblast and human gingival fibroblast cells. In addition, the synthesis of function-related proteins and gene expression levels in both MC3T3-E1 and fibroblast cells was higher for the Col/HA coating compared with the Chi/HA coating, indicating better cellular response to the Col/HA coating.

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