Characterization of Microcystis Aeruginosa immobilized in complex of PVA and sodium alginate and its application on phosphorous removal in wastewater
来源期刊：中南大学学报(英文版)2015年第1期
论文作者：LI Fei(李飞) MAO Wen-juan(毛文娟) LI Xue(李雪) WANG Xiao-yu(王晓钰) XIAO Zhi-hua(肖智华) ZHOU Yao-yu(周耀渝) ZENG Guang-ming(曾光明)
文章页码：95 - 102
Key words：eutrophication; immobilized Microcystis Aeruginosa (MA); bioactivity; phosphorous removal
Abstract: Based on ecological niche theory, Microcystis Aeruginosa (MA) immobilized in the complex of polyvinyl alcohol (PVA) and sodium alginate (SA) crosslinked by CaCl2, was treated as a new kind of special species, and its properties were investigated. Chlorophyll a was used to characterize the bioactivity of the immobilized MA. Results reveal that the gel beads have mechanical strength and chemical stability even under non-sterile harsh conditions, which may be attributed to the rarely seen structure (including three different layers: dense surface, tubular-shaped divergent structure and honeycomb crystal lattice layer) of the immobilized MA determined by scanning electron microscope (SEM). SEM also displays that more quantity of MA is attached to the inwall after cultivation, which demonstrates that the MA within beads maintains high bioactivity. Removal capacities on phosphorous (P) removal in wastewater in the presence and absence of the BG-11 medium were examined, and the removal ratios are 80.3% and 76.7%, respectively, which indicates that the beads without providing ample nutrients still have high capacity of P removal. In addition, control experiment, utilizing polyvinyl alcohol and sodium alginate (PVA-SA) beads without immobilized MA, demonstrates that MA within beads plays the key role in absorbing P.
LI Fei(李飞)1, 2, MAO Wen-juan(毛文娟)1, 2, LI Xue(李雪)3, WANG Xiao-yu(王晓钰)1, 2, XIAO Zhi-hua(肖智华)1, 2, ZHOU Yao-yu(周耀渝)1, 2, ZENG Guang-ming(曾光明)1, 2
(1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;
2. Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education
(Hunan University), Changsha 410082, China;
3. Department of Bioengineering and Environmental Science, Changsha University, Changsha 410003, China)
Abstract:Based on ecological niche theory, Microcystis Aeruginosa (MA) immobilized in the complex of polyvinyl alcohol (PVA) and sodium alginate (SA) crosslinked by CaCl2, was treated as a new kind of special species, and its properties were investigated. Chlorophyll a was used to characterize the bioactivity of the immobilized MA. Results reveal that the gel beads have mechanical strength and chemical stability even under non-sterile harsh conditions, which may be attributed to the rarely seen structure (including three different layers: dense surface, tubular-shaped divergent structure and honeycomb crystal lattice layer) of the immobilized MA determined by scanning electron microscope (SEM). SEM also displays that more quantity of MA is attached to the inwall after cultivation, which demonstrates that the MA within beads maintains high bioactivity. Removal capacities on phosphorous (P) removal in wastewater in the presence and absence of the BG-11 medium were examined, and the removal ratios are 80.3% and 76.7%, respectively, which indicates that the beads without providing ample nutrients still have high capacity of P removal. In addition, control experiment, utilizing polyvinyl alcohol and sodium alginate (PVA-SA) beads without immobilized MA, demonstrates that MA within beads plays the key role in absorbing P.
Key words:eutrophication; immobilized Microcystis Aeruginosa (MA); bioactivity; phosphorous removal
