Simultaneous determination mercury species of Su-He-Xiang-Wan in rat tissues by HPLC-CVG-AFS
来源期刊：中南大学学报(英文版)2013年第4期
论文作者：WANG Wei-ping(王伟萍) ZhANG Ming-yue(张明玥) AZIZ Mohemmat(艾则孜) GE Jing(葛婧) LIANG Yi-zeng(梁逸曾)
文章页码：894 - 901
Key words：HPLC-CV-AFS; mercury species; rat tissue; traditional Chinese medicine; Su-He-Xiang-Wan (SHXW)
Abstract: A simple and sensitive high performance liquid chromatography-chemical vapour generation-atom fluorescent spectrometry (HPLC-CVG-AFS) method was developed and validated for simultaneous determination mercury species in Su-He-Xiang-Wan (SHXW) and in tissues of rats, respectively. The species of mercury were separated by a Venusil MP-C18 (5 μm, 150 mm×4.6 mm) column with the optimized mobile phase containing 5% (w/v) acetonitrile, 0.01 mol/L L-cysteine and 0.06 moL/L ammonium acetate. The tissues of rats were freeze-dried after giving the medicine for 10 d, and then added into the solution containing 10% (w/v) HCl, 1% (w/v) sulfocarbamide and 0.15% (w/v) KCl for increasing extraction rate. The resolutions of Hg2+, MeHg and EtHg were 1.5 and 2.9, respectively. The detection limits of Hg2+, MeHg and EtHg were 2.0, 1.0 and 0.9 ng/mL, respectively. The relative standard deviation (RSD) of inter- and intra-day precisions ranged from 1.56% to 2.86%. The recovery rates of three different adding level were 87%-101% (n=6), and the RSDs were smaller than 8.2%. The results show that no MeHg and EtHg were detected in rat tissues. Only soluble mercury (Hg2+) was determined for the mercury species of SHXW in rat tissues.
WANG Wei-ping(王伟萍)1,2, ZhANG Ming-yue(张明玥)2, AZIZ Mohemmat(艾则孜)2, GE Jing(葛婧)2, LIANG Yi-zeng(梁逸曾)1
(1. Research Center of Modernization of Chinese Medicines, College of Chemistry and Chemical Engineering,
Central South University, Changsha 410083, China;
2. Xinjiang Uygur Autonomous Region Institute for Food and Drug Control, Urumqi 830004, China)
Abstract:A simple and sensitive high performance liquid chromatography-chemical vapour generation-atom fluorescent spectrometry (HPLC-CVG-AFS) method was developed and validated for simultaneous determination mercury species in Su-He-Xiang-Wan (SHXW) and in tissues of rats, respectively. The species of mercury were separated by a Venusil MP-C18 (5 μm, 150 mm×4.6 mm) column with the optimized mobile phase containing 5% (w/v) acetonitrile, 0.01 mol/L L-cysteine and 0.06 moL/L ammonium acetate. The tissues of rats were freeze-dried after giving the medicine for 10 d, and then added into the solution containing 10% (w/v) HCl, 1% (w/v) sulfocarbamide and 0.15% (w/v) KCl for increasing extraction rate. The resolutions of Hg2+, MeHg and EtHg were 1.5 and 2.9, respectively. The detection limits of Hg2+, MeHg and EtHg were 2.0, 1.0 and 0.9 ng/mL, respectively. The relative standard deviation (RSD) of inter- and intra-day precisions ranged from 1.56% to 2.86%. The recovery rates of three different adding level were 87%-101% (n=6), and the RSDs were smaller than 8.2%. The results show that no MeHg and EtHg were detected in rat tissues. Only soluble mercury (Hg2+) was determined for the mercury species of SHXW in rat tissues.
Key words:HPLC-CV-AFS; mercury species; rat tissue; traditional Chinese medicine; Su-He-Xiang-Wan (SHXW)
