A homogeneous DNA assay by recovering inhibited emission of rare earth ions-doped upconversion nanoparticles
来源期刊：Journal of Rare Earths2019年第1期
论文作者：Yingzhu Zhou Yinghui Chen Hao He iayan Liao Hien T.T.Duong Maryam Parviz Dayong Jin
文章页码：11 - 18
摘 要：Robust and easy-to-use kits specific for a particular DNA sequence are desirable for early detection of diseases. However, the major challenge with these tests is often the background fluorescence artifacts arising from biological species due to employing UV and visible range of light. Here, we have reported a near-infrared （NIR） fluorescence "turn-on" kit based on rare earth ions doped nanoparticles, upconversion nanoparticles （UCNPs）, and gold nanoparticles （AuNPs）, which forms a fluorescence-quencher pair,brought together by a hairpin structure through the formation of double-stranded DNA （dsDNA）, with quenched upconversion luminescence. In the presence of analytes, the molecular beacon opens to push AuNPs away from UCNPs, with a distance longer than the efficient quenching distance, so that the inhibited upconversion emission will be restored. We demonstrated that this assay provides a homogeneous, facile, simple and highly selective HIV-1 based DNA detection system with restore efficiency up to 85%, and the detection limit of 5 nm.
Yingzhu Zhou1，Yinghui Chen1,2，Hao He1,2，iayan Liao1，Hien T.T.Duong3，Maryam Parviz1,2，Dayong Jin1,2
1. Institute for Biomedical Materials and Devices, School of Mathematical and Physical Sciences, Faculty of Science, University of Technology Sydney2. ARC Research Hub for Integrated Device for End-User Analysis at Low-levels, Faculty of Science, University of Technology Sydney3. The Faculty of Pharmacy, The University of Sydney
摘 要：Robust and easy-to-use kits specific for a particular DNA sequence are desirable for early detection of diseases. However, the major challenge with these tests is often the background fluorescence artifacts arising from biological species due to employing UV and visible range of light. Here, we have reported a near-infrared （NIR） fluorescence "turn-on" kit based on rare earth ions doped nanoparticles, upconversion nanoparticles （UCNPs）, and gold nanoparticles （AuNPs）, which forms a fluorescence-quencher pair,brought together by a hairpin structure through the formation of double-stranded DNA （dsDNA）, with quenched upconversion luminescence. In the presence of analytes, the molecular beacon opens to push AuNPs away from UCNPs, with a distance longer than the efficient quenching distance, so that the inhibited upconversion emission will be restored. We demonstrated that this assay provides a homogeneous, facile, simple and highly selective HIV-1 based DNA detection system with restore efficiency up to 85%, and the detection limit of 5 nm.
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