Colloidal(Gd0.98Nd0.02)2O3 nanothermometers operating in a cell culture medium within the first and second biological windows
来源期刊：Journal of Rare Earths2020年第5期
论文作者：M.L.Debasu H.Oliveira J.Rocha L.D.Carlos
文章页码：483 - 491
摘 要：Non-contact,self-referenced and near-infrared luminescent nanothermometers have been recognized as emerging tools in the fields of nanomedicine and nanotechnology due to their great capability of precise temperature readout at the nanoscale and real-time deep-tissue imaging.However,the development of multifunctional and biocompatible luminescent nanothermometers operating within the optically transparent biological windows with high thermal sensitivity（>2.0%/K） remains challenging.Here,we present(Gd0.98Nd0.02)2 O3 nanothermometers operated effectively within the first and second biological windows upon continuous-wave laser diode excitation at 808 nm.Ratiometric thermometric parameters are defined by the relative changes in the emission intensities originating from the two Stark components of the 4 F3/2 level（R2 and R1） to the 4 I9/2（900-1000 nm）,4 I11/2（1035-1155 nm） and 4 I13/2（1300-1450 nm） multiplets.The thermo metric parameters are evaluated for colloidal samples in a cell culture medium and powder samples,and the highest thermal sensitivity（2.18%/K at 298 K） is attained for the former in the first biological window（both the excitation and emission in the 800-965 nm range）.The repeatability and temperature uncertainty are 99% and 1.2 K,respectively.The nanothermometers are biocompatible with human MNT-1 melanoma and HaCaT cells for 24 h of exposure and nanoparticle concentration up to 0.400 mg/mL,showing their potential for applications in nanomedicine,e.g.,intracellular imaging and temperature mapping.
M.L.Debasu1,2,3，H.Oliveira4，J.Rocha2，L.D.Carlos1
1. Department of Physics,CICECO-Aveiro Institute of Materials,Universidade de Aveiro2. Department of Chemistry,CICECO-Aveiro Institute of Materials,Universidade de Aveiro3. Center for High Technology Materials,University of NewMexico,NM4. Department of Biology,CESAM,Laboratory of Biotechnology and Cytomics,Universidade de Aveiro
摘 要：Non-contact,self-referenced and near-infrared luminescent nanothermometers have been recognized as emerging tools in the fields of nanomedicine and nanotechnology due to their great capability of precise temperature readout at the nanoscale and real-time deep-tissue imaging.However,the development of multifunctional and biocompatible luminescent nanothermometers operating within the optically transparent biological windows with high thermal sensitivity（>2.0%/K） remains challenging.Here,we present(Gd0.98Nd0.02)2 O3 nanothermometers operated effectively within the first and second biological windows upon continuous-wave laser diode excitation at 808 nm.Ratiometric thermometric parameters are defined by the relative changes in the emission intensities originating from the two Stark components of the 4 F3/2 level（R2 and R1） to the 4 I9/2（900-1000 nm）,4 I11/2（1035-1155 nm） and 4 I13/2（1300-1450 nm） multiplets.The thermo metric parameters are evaluated for colloidal samples in a cell culture medium and powder samples,and the highest thermal sensitivity（2.18%/K at 298 K） is attained for the former in the first biological window（both the excitation and emission in the 800-965 nm range）.The repeatability and temperature uncertainty are 99% and 1.2 K,respectively.The nanothermometers are biocompatible with human MNT-1 melanoma and HaCaT cells for 24 h of exposure and nanoparticle concentration up to 0.400 mg/mL,showing their potential for applications in nanomedicine,e.g.,intracellular imaging and temperature mapping.
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