Fractionation and characterization of polysaccharides from cyanobacteriumSpirulina(Arthrospira)maxima in nitrogen-limited batch culture

来源期刊：中南大学学报(英文版)2002年第2期

论文作者：聂珍媛 夏金兰 J．M．LEVERT

文章页码：81 - 86

Key words：characterization; cyanobacterium; extraction; fractionation; gas-liquid chromatography ;polysaccharides ; Spirulina(Arthrospira)maxima

Abstract: By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina(Arthrospira)maximathat can be bio-functionally healthy components were fractionated in four parts including the culture medium (PSCM), the external layers of the cell (PSEL), the cell wall (PSCW)and storage granules (PSSG). The four fractionated parts of polysaccharides were characterized by gas-liquid chromatography (GLC) equipped with FID and fused-silica capillary column (15 m×0.53 mm i. d.). The contents of PSCM, PSEL, PSCW and PSSGwere about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose was almost the only monosaccharide in PSSG and PSCW and most predominant in PSEL, while in PSCM, xylose, rhamnose and glucose were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugarswere found in PSEL and PSCM. These data are valuable for the selective productions of high-added value sugars fromSpirulina.