Characteristics of magnetic Fe3O4 nanoparticles encapsulated with human serum albumin
来源期刊:中南大学学报(英文版)2006年第1期
论文作者:何捍卫 刘红江 周科朝 王维 容鹏飞
文章页码:6 - 11
Key words:Fe3O4 nanoparticle; human serum albumin; chemical precipitation method; magnetic resonance imaging contrast agent
Abstract: Magnetic nanoparticles (Fe3O4) were prepared by chemical precipitation method using Fe2+and Fe3+ salts with sodium hydroxide in the nitrogen atmosphere. Fe3O4 nanoparticles were coated with human serum albumin(HSA) for magnetic resonance imaging as contrast agent. Characteristics of magnetic particles coated or uncoated were carried out using scanning electron microscopy and X-ray diffraction. Zeta potentials, package effects and distributions of colloid particles were measured to confirm the attachment of HSA on magnetic particles. Effects of Fe3O4 nanoparticles coated with HSA on magnetic resonance imaging were investigated with rats. The experimental results show that the adsorption of HSA on magnetic particles is very favorable to dispersing of magnetic Fe3O4 particles, while the sizes of Fe3O4 particles coated are related to the molar ratio of Fe3O4 to HSA. The diameters of the majority of particles coated are less than 100 nm. Fe3O4 nanoparticle coated with HSA has a good biocompatibility and low toxicity. This new contrast agent has some effects on the nuclear magnetic resonance imaging of liver and the lowest dosage is 20μmol/kg for the demands of diagnosis.