Extraction process of chlorogenic acid in flos lonicerae by enzymatic treatment

来源期刊：中南大学学报(英文版)2002年第4期

论文作者：刘佳佳 赵国玲 王晖 章晓骅

文章页码：246 - 249

Key words：flos lonicerae; chlorogenic acid; enzymatic treatment; extract process

Abstract: A newmethod of extracting chlorogenic acid from flos lonicerae, and treating the materials with enzyme before being extracted by ethanol is developed, and the optimum conditions are also investigated in detail. Three importantfactors, enzyme dosage, treatmenttime and treatmenttemperature are adapted tooptimize the extraction process. The experimental results showthatthe extractyield of flos lonicerae and chlorogenic acid can be obviously increased bythe cellulase treatment, 61.5 mg chlorogenic acid is obtained from1.00 gflos lonicerae at most. The optimal temperature of enzymatic treatment is 40-50℃. Compared with the use of single cellulase, the combined treatment of cellulase and pectinase increase the extract yield obviously but fail to improve that of chlorogenic acid.