Biocompatibility evaluation in vitro. Part II: Functional expression of human and animal osteoblasts on the biomaterials

来源期刊：中南大学学报(英文版)2001年第2期

论文作者：RUAN Jian-ming GRANT M. Helen

文章页码：75 - 82

Key words：biocompatibility; DNA synthesis; collagen protein incorporation

Abstract: DNA synthesis and collagen formations on the implantmaterial by cell culture in vitro are the most important phenotypical expression to estimate the biocompatibility. In this part, DNA synthesis and collagen formation on implantmaterials were quantitatively and qualitatively estimated by radioactive is otope H+-thymi-dine to incorporate into DNA chains, H+-proline to incorporate into type I collagen proteins followed by scintillation counting and antibody-antigen immunocytochemistry staining, respectively. Research results demonstrate that hydroxyapatite (HA) stimulates DNA synthesis and collagen formation on the material whereas this stimulation is restricted by adding spinel to the materials. There are statistical differences between the influences of material components on both DNA synthesis and collagen formation. It is supposed that porousmaterials can supply more platforms for cell anchoring, and more DNA and collagen are synthesised on the porous materials. Immersion in culture medium results in new HA crystal formation on the porous HA materials.