Enhanced Performance of Osteoblasts by Silicon Incorporated Porous TiO₂ Coating

Quanming Wang 1),Hongjie Hu 2),Yuqing Qiao 2),Zhengxiang Zhang 1) and Junying Sun 1) 1) Orthopaedic Department,The First Affiliated Hospital of Soochow University,188 Shizi Street,Suzhou 215006,China 2) State Key Laboratory of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China

摘 要：Silicon （Si） incorporated porous TiO 2 coating （Si-TiO 2） prepared on titanium （Ti） by micro-arc oxidation （MAO） technique was demonstrated to be cytocompatible in previous studies.In view of the potential clinical applications,a detailed in vitro study of the biological activity of Si-TiO 2 coating,in terms of osteoblast （MC3T3-E1 cells） morphology,proliferation,differentiation and mineralization was performed.Immunofluorescent staining indicated that cells seeded on the Si-TiO 2 coating showed improved adhesion with developing mature cytoskeletons,which contained numerous distinct and well-defined actin stress fibers in the cell membranes compared with those on the TiO 2 coating and Ti plate.Results from proliferation assay showed that the proliferation rate of cells seeded on the Si-TiO 2 coating was significantly faster than that on the TiO 2 coating and Ti plate.Furthermore,the analysis of osteogenic gene expression demonstrated that the Si-TiO 2 coating stimulated the expression of osteoblast-related genes and promoted differentiation and mineralization of MC3T3-E1 cells.In addition,the Si-TiO 2 coating differentially regulated Wnt signaling pathway by up-regulating the expression of low-density lipoprotein （LDL） receptor-related protein 5 （Lrp5）,and downregulating the expression of Dickkopf-1 （Dkk1）.All together,these results indicate that the investigated titanium with Si-TiO 2 coating is biocompatible and a good candidate material used as implants.

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