Cytocompatibility of Self-assembled Hydrogel from IKVAV-containing Peptide Amphiphile with Neural Stem Cells
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2009年第5期
论文作者：宋玉林
文章页码：753 - 756
摘 要：Neural Stem Cells （NSCs） were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile （IKVAV-PA） for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional （3D） hydrogels （Experimental Group, EG） or surface of coverslips （Control Group, CG）, double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG （P<0.05）. The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.
宋玉林
Department of Orthopaedics, the Second Affiliated Hospital of Nanchang University
摘 要：Neural Stem Cells （NSCs） were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile （IKVAV-PA） for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional （3D） hydrogels （Experimental Group, EG） or surface of coverslips （Control Group, CG）, double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG （P<0.05）. The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.
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