缺氧胁迫小细胞团法选育高产黄酮苷悬浮细胞系

来源期刊：中南大学学报(自然科学版)2001年第1期

论文作者：刘佳佳 郭勇 郑穗平 张剑锋

文章页码：41 - 45

关键词：缺氧胁迫;小细胞团;黄酮苷;悬浮细胞系

Key words：hypoxia stress; small cell groups; flavonol glycoside; cell line for suspension culture

摘    要：以银杏优良品种的种苗诱导愈伤组织,考察了B₅,SH,MS,White 4种培养基对银杏愈伤组织生长性状和黄酮苷合成能力的影响,采用缺氧胁迫小细胞团法从愈伤组织中筛选高产黄酮苷的细胞系,并对细胞系在悬浮培养时的条件进行了优化.结果表明:TZG-1培养周期18 d,生长指数为4.12,黄酮苷含量为1.25%,比愈伤组织提高了257.1%;MS培养基有利于形成质地松软的愈伤组织,当培养基中NH⁺⁴与NO^-3的浓度比为20.6/39.4或10.3/29.1,蔗糖质量浓度为30 g/L或40 g/L,pH值为5.6~6.0时,有利于细胞生长和黄酮苷合成.

Abstract: The effects of media (B₅, SH, white, MS) on the growth and synthesis of flavonol glycoside of calli introduced from seedling of Ginkgo biloba, the cell lines for suspension culture selected by hypoxia stress for small celgroups, and the factors influencing cell growth and synthesis of flavonol glycoside in suspension culturewere studied. Thresults showthatMSmedia are suitable to introducing callus ofGinkgo bilobaL, cell line selected has 4.12 of growth index and 1.25%(dry mass fraction of cells) of flavonol glycoside that is enhanced by 257.1% compared with calli, an thec(NH⁺⁴)/c(NO^-3)(20.6/39.4 or 10.3/29·1), sucrose content (30~40 g/L), pH(5.6~6.0) are beneficial t cell growth and synthesis of flavonol glycoside.