Microwave-assisted extraction of polysaccharides from solanum nigrum^①

来源期刊：中南大学学报(英文版)2005年第5期

论文作者：陈晓青 刘覃 蒋新宇 曾帆

文章页码：556 - 560

Key words：solanum nigrum; polysaccharides; structure; microwave-assisted extraction

Abstract: The microwave-assisted method was used to extract polysaccharides from solanum nigrum. The optimum experimental parameters, mechanism of the extraction and the effect of microwave-assisted extraction process on the structures of polysaccharides were investigated. The extract was analyzed by the modified phenol-sulfuric acid method at 490 nm. The optimum experimental parameters were obtained by orthogonal experiments as follows: extraction time 15 min, microwave radiation power 455 W and the process ratio of materials mass to solvent volume 1∶20. The results show that compared with the conventional reflux extraction, the microwave-assisted extraction has a higher yield in shorter time, with no effect on the finally obtained polysaccharides as seen from the FT-IR spectra. The scanning electron microscopy images reveal that the mechanism of the extraction is related to the structural changes of the plant cells in different extracting conditions.