A microfluidic approach for development of hybrid collagen-chitosan extracellular matrix-like membranes for on-chip cell cultures
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2021年第4期
论文作者：Erica Rosella Nan Jia Diego Mantovani Jesse Greener
摘 要：To advance organ-on-a-chip development and other areas befitting from physiologically-relevant biomembranes, a microfluidic platform is presented for synthesis of biomembranes during gelation and investigation into their role as extracellular matrix supports. In this work, high-throughput studies of collagen, chitosan, and collagen-chitosan hybrid biomembranes were carried out to characterize and compare key properties as a function of the applied hydrodynamic conditions during gelation. Specifically, depending on the biopolymer material used, varying flow conditions during biomembrane gelation caused width, uniformity, and swelling ratio to be differently affected and controllable. Finally, cell viability studies of seeded fibroblasts were conducted, thus showing the potential for biological applications.
Erica Rosella1,2，Nan Jia1，Diego Mantovani2,3，Jesse Greener1,3
1. Laboratory, Department of Chemistry, Laval University2. Laboratory of Biomaterials and Bioengineering, CRC-1, Dept.Min-Met-Materials Eng & CHU de Quebec, Research Center, Laval University3. CHU de Quebec Research Centre, Laval University
摘 要：To advance organ-on-a-chip development and other areas befitting from physiologically-relevant biomembranes, a microfluidic platform is presented for synthesis of biomembranes during gelation and investigation into their role as extracellular matrix supports. In this work, high-throughput studies of collagen, chitosan, and collagen-chitosan hybrid biomembranes were carried out to characterize and compare key properties as a function of the applied hydrodynamic conditions during gelation. Specifically, depending on the biopolymer material used, varying flow conditions during biomembrane gelation caused width, uniformity, and swelling ratio to be differently affected and controllable. Finally, cell viability studies of seeded fibroblasts were conducted, thus showing the potential for biological applications.
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