Antibacterial behavior and related mechanisms of martensitic Cu-bearing stainless steel evaluated by a mixed infection model of Escherichia coli and Staphylococcus aureus in vitro
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2021年第3期
论文作者：Mingjun Li Li Nan Chunyong Liang Ziqing Sun Lei Yang Ke Yang
文章页码：139 - 147
摘 要：Escherichia coli（E.coli） and Staphylococcus aureus（S.aureus） are the most typical pathogenic bacteria with a significantly high risk of bio-contamination,widely existing in hospital and public places.Recent studies on antibacterial materials and the related mechanisms have attracted more interests of researchers.However,the antibacterial behavior of materials is usually evaluated separately on the single bacterial strain,which is far from the practical condition.Actually,the interaction between the polymicrobial communities can promote the growing profile of bacteria,which may weaken the antibacterial effect of materials.In this work,a 420 copper-bearing martensitic stainless steel（420 CuSS） was studied with respect to its antibacterial activity and the underlying mechanism in a co-culturing infection model using both E.coli and S.au reus.Observed via plating and counting colony forming units（CFU）,Cu releasing,and material characterization,420 CuSS was proved to present excellent antibacterial performance against the mixed bacteria with an approximately 99.4 % of antibacterial rate.In addition,420 CuSS could effectively inhibit the biofilm formation on its surfaces,resulting from a synergistic antibacterial effect of Cu ions,Fe ions,reactive oxygen species（ROS）,and proton consumption of bacteria.
Mingjun Li1,2，Li Nan2，Chunyong Liang1，Ziqing Sun2，Lei Yang1,3，Ke Yang2
1. Center for Health Science and Engineering, School of Materials Science and Engineering, Hebei University of Technology2. Institute of Metal Research, University of Chinese Academy of Sciences3. International Research Center for Translational Orthopaedics (IRCTO)
摘 要：Escherichia coli（E.coli） and Staphylococcus aureus（S.aureus） are the most typical pathogenic bacteria with a significantly high risk of bio-contamination,widely existing in hospital and public places.Recent studies on antibacterial materials and the related mechanisms have attracted more interests of researchers.However,the antibacterial behavior of materials is usually evaluated separately on the single bacterial strain,which is far from the practical condition.Actually,the interaction between the polymicrobial communities can promote the growing profile of bacteria,which may weaken the antibacterial effect of materials.In this work,a 420 copper-bearing martensitic stainless steel（420 CuSS） was studied with respect to its antibacterial activity and the underlying mechanism in a co-culturing infection model using both E.coli and S.au reus.Observed via plating and counting colony forming units（CFU）,Cu releasing,and material characterization,420 CuSS was proved to present excellent antibacterial performance against the mixed bacteria with an approximately 99.4 % of antibacterial rate.In addition,420 CuSS could effectively inhibit the biofilm formation on its surfaces,resulting from a synergistic antibacterial effect of Cu ions,Fe ions,reactive oxygen species（ROS）,and proton consumption of bacteria.
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