Extraction optimization of coumarins from radix angelicae pubescentis by HPLC-DAD coupled with uniform design

来源期刊：中南大学学报(英文版)2006年第2期

论文作者：郭方道 黄兰芳 周火飞 江秋菊

文章页码：156 - 159

Key words：radix angelicae pubescentis(Duhuo); couamrins; uniform design; high performance liquid chromata graphy-diode array detector(HPLC-DAD)

Abstract: Uniform design was used to optimize extraction condition of direct refluence extraction of coumarins from the Chinese traditional medicine of radix angelicae pubescentis(Duhuo); the sum peak area of coumarins separated with high performance liquid chromatography(HPLC) at detection wavelength of 320 nm was considered as detection index, two factors of solvent concentration and extraction time were mainly studied at extraction temperature of 85℃and a volume ratio of solvent to sample of 10∶1. Optimal subclass, quadric polynomial step by step aggression and neural network method were applied to process the experimental results. The results show that the first and second methods give the same factors combination (concentration of ethanol: 95%, extraction time: 3.6 h) and the second method is much better than the first one. The extraction model is consequently developed.