杜仲中松脂醇二葡萄糖甙的提纯
来源期刊：中南大学学报(自然科学版)2003年第3期
论文作者：陈晓青 李宇萍 彭密军 王玉林 出山武
文章页码：262 - 265
关键词：杜仲; PDG;大孔树脂;提取;测定
Key words：eucommia ulmoide oliver; PDG; macro-reticular resins; extraction; determination
摘 要：对杜仲中活性成分松脂醇二葡萄糖甙(Pinoresinol DiGlucoside ,即PDG)提取、纯化工艺进行了研究,并使用高效液相色谱法测定PDG含量.结果表明:杜仲粉用体积分数为60%的乙醇水溶液,60℃,提取2次,每次1 h,PDG提取率可达90%以上.分别采用4种大孔树脂对提取液进行分离纯化处理,以不同浓度的乙醇水溶液进行梯度洗脱,发现S型树脂对此种活性成分选择性好、吸附量大,用体积分数为40%的乙醇水溶液洗脱可将其完全解吸附,PDG的收率达45.2%.在C18色谱柱上,以体积分数为26%的甲醇水溶液为流动相,流速为1.0 mL/min,在λ=228 nm处,对杜仲原料中PDG进行了测定,结果表明炮制后的杜仲皮的PDG质量分数较高,为0.497%.
Abstract: A technological process for the extraction and purification of pinoresinol diglucoside (PDG ) in eucommia ulmoide oliver was studied andHPLC method for determination was used. The powder of eucommia was extracted twice with 60%(φ) ethanol-water solution at 60℃, for 60 min each time. The eucommia solution was subjected to four different macro-reticular resins column chromatography, eluted with ethanol-water solution by gradient elution respectively. The results show that S resin is good because of high selectivity, high capability of adsorbability and easiness of being eluted; the extraction ratio of PDG reaches 90%, the PDG recovery rate of purification process is 44.5%. The PDG of eucommia ulmoide oliver was determined by HPLC at 228 nm under the conditions :ODS-C18 column, 26%(φ)MeOH-H2O as mobile phase. The results show that the content of PDG of the roasted bark is the highest.
基金信息：湖南省科研计划项目
