A graphene-laminated electrode with high glucose oxidase loading for highly-sensitive glucose detection
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2021年第7期
论文作者：Yabin Hao Minghe Fang Chuan Xu Zhe Ying Han Wang Rui Zhang Hui-Ming Cheng You Zeng
文章页码：57 - 63
摘 要：Graphene oxide（GO） has received considerable attention for glucose detection because of high surface area, abundant functional groups, and good biocompatibility. Defects and functional groups of the GO are beneficial to immobilization of glucose oxidase（GOD）, but sacrificing electron-transfer capability for highly-sensitive detection. In order to obtain high GOD loading and highly-sensitive detection of biosensors, we first designed and fabricated a graphene-laminated electrode by combining GO and edgefunctionalized graphene（FG） layers together onto glassy-carbon electrode. The graphene-laminated electrodes exhibited faster electron transfer rate, higher GOD loading of 3.80 × 10-9 mol·cm-2, and higher detection sensitivity of 46.71 μA·mM-1·cm-2 than other graphene-based biosensors reported in literature. Such high performance is mainly attributed to the abundant functional groups of GO, high electrical conductivity of FG, and strong interactions between components in the graphene-laminated electrodes.By virtue of their high enzyme loading and highly-sensitive detection, the graphene-laminated electrodes show great potential to be widely used as high-performance biosensors in the field of medical diagnosis.
Yabin Hao1,2，Minghe Fang1,2，Chuan Xu1，Zhe Ying1，Han Wang1,2，Rui Zhang3，Hui-Ming Cheng1,2,4，You Zeng1,2
1. Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences2. School of Materials Science and Engineering, University of Science and Technology of China3. Liaoning Cancer Hospital and Institute4. Shenzhen Geim Graphene Center, Tsinghua-Berkeley Shenzhen Institute
摘 要：Graphene oxide（GO） has received considerable attention for glucose detection because of high surface area, abundant functional groups, and good biocompatibility. Defects and functional groups of the GO are beneficial to immobilization of glucose oxidase（GOD）, but sacrificing electron-transfer capability for highly-sensitive detection. In order to obtain high GOD loading and highly-sensitive detection of biosensors, we first designed and fabricated a graphene-laminated electrode by combining GO and edgefunctionalized graphene（FG） layers together onto glassy-carbon electrode. The graphene-laminated electrodes exhibited faster electron transfer rate, higher GOD loading of 3.80 × 10-9 mol·cm-2, and higher detection sensitivity of 46.71 μA·mM-1·cm-2 than other graphene-based biosensors reported in literature. Such high performance is mainly attributed to the abundant functional groups of GO, high electrical conductivity of FG, and strong interactions between components in the graphene-laminated electrodes.By virtue of their high enzyme loading and highly-sensitive detection, the graphene-laminated electrodes show great potential to be widely used as high-performance biosensors in the field of medical diagnosis.
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