Ce4+-Induced Apoptosis of Taxus cuspidata Cells in Suspension Culture
来源期刊：JOURNAL OF RARE EARTHS2002年第2期
论文作者：元英进 马振毅 葛志强 胡宗定 王艳东
Key words：rare earths; cerium; apoptosis; Taxus cuspidata; cell suspension culture;
Abstract: The standard detection hallmarks of apoptosis of Taxus cuspidata cells in suspension culture with Ce4+ were studied. The condensation and margination of chromatin were observed under the electron microscopy. DNA fragmentation ranged "DNA ladder" on agarose gel electrophoresis. TdT-mediated dUTP nick end labeling (TUNEL) analysis of the cells reveals that the nuclear DNA strand breaks can be identified by labeling free 3′-OH termini. These results suggest that Ce4+ can induce apoptosis of Taxus cuspidata cells and also indicate that there is a certain relationship between apoptosis and secondary metabolite product-Taxol.
元英进1，马振毅1，葛志强1，胡宗定1，王艳东1
(1.Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China)
Abstract:The standard detection hallmarks of apoptosis of Taxus cuspidata cells in suspension culture with Ce4+ were studied. The condensation and margination of chromatin were observed under the electron microscopy. DNA fragmentation ranged "DNA ladder" on agarose gel electrophoresis. TdT-mediated dUTP nick end labeling (TUNEL) analysis of the cells reveals that the nuclear DNA strand breaks can be identified by labeling free 3′-OH termini. These results suggest that Ce4+ can induce apoptosis of Taxus cuspidata cells and also indicate that there is a certain relationship between apoptosis and secondary metabolite product-Taxol.
Key words:rare earths; cerium; apoptosis; Taxus cuspidata; cell suspension culture;
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