Enzymatic detection of ethanol based on H2O2-sensitive quantum dots
来源期刊：中南大学学报(英文版)2012年第11期
论文作者：FU Xin(傅昕) ZHANG He(张何) XIAO Jun-ping(肖俊平) LIU Su-qin(刘素琴)
文章页码：3040 - 3045
Key words：enzymatic detection; CdSe/ZnS quantum dots; alcohol oxidase; hydrogen peroxide; photoluminescence
Abstract: A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H2O2. This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx) which, on oxidation of ethanol, produces H2O2. The method has higher sensitivity, wider analytical range (0.1-8 mmol/L), and a lower detection limit (0.05 mmol/L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.
FU Xin(傅昕)1,2, ZHANG He(张何)1, XIAO Jun-ping(肖俊平)1, LIU Su-qin(刘素琴)2
(1. School of Chemistry and Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China;
2. Department of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China)
Abstract:A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H2O2. This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx) which, on oxidation of ethanol, produces H2O2. The method has higher sensitivity, wider analytical range (0.1-8 mmol/L), and a lower detection limit (0.05 mmol/L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.
Key words:enzymatic detection; CdSe/ZnS quantum dots; alcohol oxidase; hydrogen peroxide; photoluminescence
