Synthesis and Evaluation of Novel Chitosan Derivatives for Gene Delivery
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2015年第4期
论文作者：付芳 LIU Xiong 吕波 QU Guoyi ZHENG Hua HUANG Zhijun XU Peihu XU Haixing YIN Yihua LIU Xia ZHANG Xueqiong
文章页码：852 - 858
摘 要：A series of novel water soluble chitosan derivatives as gene vectors was synthesized. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilizing agarose gel electrophoresis, particle size analysis, zeta potential measurement and scanning electron microscopy. Furthermore, cytotoxicity of chitosan derivatives and transfection efficiency of polyplexes were evaluated in vitro. The experimental results showed that the novel chitosan derivatives had lower cytotoxicity, good DNA condensation, and higher transfection efficiencies compared to chitosan in both 293 T and He La cell lines. It was indicated that these chitosan derivatives were promising candidates for non-viral gene vectors.
付芳1，LIU Xiong1，吕波1，QU Guoyi2，ZHENG Hua1，HUANG Zhijun1，XU Peihu1，XU Haixing1，YIN Yihua1，LIU Xia1，ZHANG Xueqiong1
1. School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology2. Luo Tian Wan Mizhai Hospital
摘 要：A series of novel water soluble chitosan derivatives as gene vectors was synthesized. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilizing agarose gel electrophoresis, particle size analysis, zeta potential measurement and scanning electron microscopy. Furthermore, cytotoxicity of chitosan derivatives and transfection efficiency of polyplexes were evaluated in vitro. The experimental results showed that the novel chitosan derivatives had lower cytotoxicity, good DNA condensation, and higher transfection efficiencies compared to chitosan in both 293 T and He La cell lines. It was indicated that these chitosan derivatives were promising candidates for non-viral gene vectors.
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