In vitro and in vivo biodegradation and biocompatibility of an MMT/BSA composite coating upon magnesium alloy AZ31
来源期刊：JOURNAL OF MATERIALS SCIENCE TECHNOLOG2020年第12期
论文作者：Jian Wang Lanyue Cui YANDe Ren Yuhong Zou Jinlong Ma Chengjian Wang Zhongyin Zheng Xiaobo Chen Rongchang Zeng Yufeng Zheng
文章页码：52 - 67
摘 要：The performance of biodegradable magnesium alloy requires special attention to rapid degradation and poor biocompatibility, which can cause the implant to fail. Here, a sodium montmorillonite（MMT）/bovine serum albumin（BSA） composite coating was prepared upon magnesium alloy AZ31 via hydrothermal synthesis, followed by dip coating. We evaluated the surface characterization and corrosion behavior in vitro, and the biocompatibility in vitro and in vivo. Biodegradation progress of the MMT-BSA coated Mg pieces was examined through hydrogen evolution, immersion tests, and electrochemical measurements in Hank’s solution. In vitro biocompatibility studies were evaluated via hemolysis tests, dynamic cruor time tests, platelet adhesion, MTT testing and live-dead stain of osteoblast cells（MC3 T3-E1）. It was found that the MMT-BSA coating had good corrosion resistance and a marked improvement in biocompatibility in comparison to bare Mg alloy AZ31. in vivo studies were carried out in rat model and the degradation was characterized by computed tomography scans. Results revealed that the MMT-BSA coated Mg alloy AZ31 implants maintained their structural integrity and slight degradation after 120 d of post-implantation. A100% survival rate for the rats was observed with no obvious toxic damages on the organs and tissues.Additionally, we proposed a sound coating formation mechanism. Considering the good corrosion protection and biocompatibility, the MMT-BSA coated Mg alloy AZ31 is a promising candidate material for biomedical implants.
Jian Wang1，Lanyue Cui2，YANDe Ren3，Yuhong Zou1，Jinlong Ma3，Chengjian Wang3，Zhongyin Zheng1，Xiaobo Chen4，Rongchang Zeng2,5，Yufeng Zheng6
1. Department of Bioengineering, College of Chemical and Biological Engineering, Shandong University of Science and Technology2. Corrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology3. Affiliated Hospital of Medical College Qingdao University4. School of Engineering, RMIT University5. School of Materials Science and Engineering, Zhengzhou University6. State Key Laboratory for Turbulence and Complex Systems and Department of Materials Science and Engineering, College of Engineering, Peking University
摘 要：The performance of biodegradable magnesium alloy requires special attention to rapid degradation and poor biocompatibility, which can cause the implant to fail. Here, a sodium montmorillonite（MMT）/bovine serum albumin（BSA） composite coating was prepared upon magnesium alloy AZ31 via hydrothermal synthesis, followed by dip coating. We evaluated the surface characterization and corrosion behavior in vitro, and the biocompatibility in vitro and in vivo. Biodegradation progress of the MMT-BSA coated Mg pieces was examined through hydrogen evolution, immersion tests, and electrochemical measurements in Hank’s solution. In vitro biocompatibility studies were evaluated via hemolysis tests, dynamic cruor time tests, platelet adhesion, MTT testing and live-dead stain of osteoblast cells（MC3 T3-E1）. It was found that the MMT-BSA coating had good corrosion resistance and a marked improvement in biocompatibility in comparison to bare Mg alloy AZ31. in vivo studies were carried out in rat model and the degradation was characterized by computed tomography scans. Results revealed that the MMT-BSA coated Mg alloy AZ31 implants maintained their structural integrity and slight degradation after 120 d of post-implantation. A100% survival rate for the rats was observed with no obvious toxic damages on the organs and tissues.Additionally, we proposed a sound coating formation mechanism. Considering the good corrosion protection and biocompatibility, the MMT-BSA coated Mg alloy AZ31 is a promising candidate material for biomedical implants.
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