The Enhancement of Osteogenesis by Scaffold Based on Mineralized Recombinant Human-like Collagen Loading with rhBMP-2
来源期刊：Journal Of Wuhan University Of Technology Materials Science Edition2009年第6期
论文作者：吴斌 郑启新
文章页码：956 - 960
摘 要：A biomimetic scaffold based on mineralized recombinant collagen, nano-hydroxyapatite/recombinant human-like collagen/poly（lactic acid） （nHA/RHLC/PLA）, was prepared with recombinant human bone morphogenic protein-2 （rhBMP-2） for improving the osteoinductive property of the scaffold. The nHA/RHLC/PLA scaffolds loaded with 10 μg rhBMP-2 and the unloaded scaffolds were implanted subcutaneously in the rat model. The osteogenetic capacity of these composites was evaluated by CT scan, ALP activity test and histological observation at 4 and 8 weeks after implantation. The experimental results indicated that the osteogenic capability of the scaffolds loaded with rhBMP-2 was superior to the unloaded scaffold. It was concluded that rhBMP-2 can enhance the osteoinductive property of the nHA/RHLC/PLA scaffold and the nHA/RHLC/PLA scaffold loaded with rhBMP-2 have the good potential of being used in bone tissue engineering.
吴斌，郑启新
Department of Orthopaedics,Union Hospital,Tongji Medical College,Huazhong University ofScience & Technology
摘 要：A biomimetic scaffold based on mineralized recombinant collagen, nano-hydroxyapatite/recombinant human-like collagen/poly（lactic acid） （nHA/RHLC/PLA）, was prepared with recombinant human bone morphogenic protein-2 （rhBMP-2） for improving the osteoinductive property of the scaffold. The nHA/RHLC/PLA scaffolds loaded with 10 μg rhBMP-2 and the unloaded scaffolds were implanted subcutaneously in the rat model. The osteogenetic capacity of these composites was evaluated by CT scan, ALP activity test and histological observation at 4 and 8 weeks after implantation. The experimental results indicated that the osteogenic capability of the scaffolds loaded with rhBMP-2 was superior to the unloaded scaffold. It was concluded that rhBMP-2 can enhance the osteoinductive property of the nHA/RHLC/PLA scaffold and the nHA/RHLC/PLA scaffold loaded with rhBMP-2 have the good potential of being used in bone tissue engineering.
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