耐高温小球藻紫外诱变育种及其耐高温性质研究

来源期刊:中南大学学报(自然科学版)2013年第3期

论文作者:夏金兰 宁进军 陈程浩 万民熙 聂珍媛

文章页码:867 - 873

关键词:紫外诱变;小球藻;耐高温性;生物柴油;烟道气

Key words:UV mutagenesis; Chloreela sorokiniana; thermotolerance; biodiesel; flue gas

摘    要:从内蒙古筛选得到1株淡水小球藻Chlorella sorokiniana CS-01,采用原生质体-紫外线诱变技术,以55 ℃高温培养为选择压力,得到1株能在该温度下存活的突变藻株,该突变藻株能够适应利用烟道气CO2培养造成的高温环境。突变藻株适应的温度范围更广(15~55 ℃),生长周期缩短,生物质产量增加;当通入装机容量为500 MW的火力发电厂烟道气,其生长周期较Chlorella sorokiniana CS-01缩短2 d,油脂质量分数提高20%。传代培养5代,生长情形相同,具有较好的遗传稳定性;该藻株具有火电厂、冶金厂、水泥厂等高排放工业位点固定烟道气CO2的应用价值,生物质经油脂提取和转酯反应可用于制取生物柴油。

Abstract: A fresh water microalga named Chlorella sorokiniana CS-01 which was isolated from Inner Mongol was mutated by UV mutagenesis of protoplasts and screened at high culture temperature (55 ℃), the mutated microalga can withstand high temperature caused by the flue gas. It has a wider growth temperature (15-55 ℃) and shorter life cycle, and a higher biomass yield. Pumping into a simulative 500 MW thermal power plant flue gas at 38 ℃, its life cycle is shortened by 2 d and the oil content was raised by 20%. This mutated microalga has good stability of heredity, and it has considerable commercial value for fixing CO2 of flue gases from high CO2 emission sites, e.g. thermo-power plants, metallurgical plants and cement plants. The biomass by oil-extraction and esterification can be used to produce biodiesel.

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