Effect of Lanthanum and Cerium on Expression of RSCU-PA-32k Gene in Saccharomyces Cerevisiae
来源期刊:JOURNAL OF RARE EARTHS2002年第6期
论文作者:刘玉鹏 刘晖 徐长法 王兰仙 任育红
Key words:rare earths; lanthanum chloride; cerium chloride; low molecular weight single-chain urokinase-type plasminogen activator; yeast;
Abstract: Lanthanum chloride can promote expression efficiency of rscu-PA-32k gene in yeast. 2 and 5 mmol·L-1 LaCl3 increase the activities of the expression product by 13% and 20% (from 14.6 to 16.5 and 17.5 U·ml-1). Cerium chloride can decrease the activity of expression product. 2 and 5 mmol·L-1 CeCl3 decreases the activities of the expression product by 21% and 33% (from 14.6 to 11.5 and 9.8 U·ml-1).
刘玉鹏1,刘晖2,徐长法2,王兰仙2,任育红3
(1.Institute of Food Science and Engineering, Yantai University, Yantai 264005, China;
2.College of Life Sciences, Peking University, Beijing 100871, China;
3.Department of Biochemistry, Yantai University, Yantai 264005, China)
Abstract:Lanthanum chloride can promote expression efficiency of rscu-PA-32k gene in yeast. 2 and 5 mmol·L-1 LaCl3 increase the activities of the expression product by 13% and 20% (from 14.6 to 16.5 and 17.5 U·ml-1). Cerium chloride can decrease the activity of expression product. 2 and 5 mmol·L-1 CeCl3 decreases the activities of the expression product by 21% and 33% (from 14.6 to 11.5 and 9.8 U·ml-1).
Key words:rare earths; lanthanum chloride; cerium chloride; low molecular weight single-chain urokinase-type plasminogen activator; yeast;
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